Microdevice Simultaneously Measures Biophysical Properties and Position of Single Cells – Scitech Daily

….a Singapore University of Technology and Design (SUTD) research team led by Associate Professor Dr Ye Ai’s developed a microfluidic impedance flow cytometry device for the lateral position measurement of single cells and particles with a novel N-shaped electrode design.

A differential current collected from N-shaped electrodes encodes the trajectory of flowing single particles. A simple analytical expression is derived for the measurement of the particle lateral position based on the relationship between the generating electrical current and the positions of the flowing particles, electrodes and microchannel, eliminating the usage of expensive high-speed camera and computationally intensive image processing or laborious manual analysis.

Principal investigator, Dr. Ai said: “Compared to previously reported impedance-based microfluidic devices for measuring the particle lateral position, we have achieved the highest measurement resolution, highest flow rate and smallest measured particle size (3.6 μm beads). On top of that, this method is more straightforward as the particle lateral position can be calculated directly from a simple analytical expression rather than using indexes, such as transit time and height of the signal peak or using linear mapping with calibration coefficients to transform the index (i.e.,